The objective of the Islet Isolation Core is to provide isolated Islets of Langerhans from rats and mice for investigators. Thanks to years of experience, the Core can predictably provide islets that are high in quantity, quality and purity.
The Core can buy animals or the investigator can provide their own, such as when special transgenic mice are required. We also provide training and expert advice. The Core can provide training not only for the islet isolation procedure but for measurement of islet viability, islet size and equivalence (IEQ).
Moreover, the Core can provide advice about a wide variety of techniques including Glucose Stimulated Insulin Secretion (GSIS), streptozocin (STZ) induction of diabetes, transplantation of islets under the kidney capsule, and islet graft retrieval. The Core can also provide advice about culturing islets, determination of DNA and insulin content, and obtaining RNA for gene expression.