The CRISPR Screen Core aims to help researchers to take advantage of the cutting-edge whole-genome CRISPR screen technology to do innovative research and discover new targets for your research projects.
The CRISPR Screen Core can help design, execute, and analyze genome-wide CRISPR/Cas9 based knockout or overexpression screen in human or mouse cells. The Core can also help generate mutant cell lines through CRISPR technology for validation and follow-up studies.
The specific services of the CRISPR Screen Core include:
- Consultation: The Core director and staff will assist the researcher in determining the feasibility of the project, selecting appropriate cell types and CRISPR libraries, and developing an overall optimal screen
Genome-wide CRISPR libraries currently available in the Core (All obtained from Addgene):
Human GeCKO V2 knockout library (1 plasmid system, 123411 gRNAs)
Human Brunello knockout library (1 plasmid system, 76441 gRNAs)
Human SAM V2 overexpression library (2 plasmid system, 70290 gRNAs)
Mouse GeCKO V2 knockout library (1 plasmid system, 130209 gRNAs)
Mouse Brie knockout library (1 plasmid system, 78637 gRNAs)
Mouse SAM V1 overexpression library (3 plasmid system, 69716 gRNAs)
- Lentiviral library production and cell transduction: The core will prepare lentiviral library propagated using the plasmid CRISPR libraries listed above, titer the lentiviral library and transduce the cells of interest.
- Genome DNA preparation and sequencing: The Core will extract genome DNA from screen samples, amplify and barcode the gRNA region and prepare Illumina sequencing library. Next generation sequencing will be done through a specialized sequencing company, and the Core will provide basic data analysis.
- Generation of validation cell line: Once a target gene of interest is determined through the screen, the core can provide service of individual gRNA cloning, lentiviral production, and mutant cell line generation for validation.